The separation of natural estrogens by countercurrent distribution.

نویسندگان

  • L L ENGEL
  • W R SLAUNWHITE
  • P CARTER
  • I T NATHANSON
چکیده

The development of fluorometric methods (l-4) for the quantitative estimation of estrogens in the small quantities present in human urine has opened the way for improvements in the procedures utilized for the extraction and separation of these compounds. A study of the partition coefficients of estrone, estradiol-178,’ and estriol in various solvent systems has been of great value in the development of a simplified extraction procedure. Counter-current distribution of the purified extracts has been found useful for the separation of the three estrogens, for their quantitative estimation, and for the evaluation of the specificity of the extraction procedure.

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 185 1  شماره 

صفحات  -

تاریخ انتشار 1950